Journal: iScience

Article Title: Cantaloupe melon genome reveals 3D chromatin features and structural relationship with the ancestral cucurbitaceae karyotype

doi: 10.1016/j.isci.2021.103696

Figure Lengend Snippet:

Article Snippet: E.Z.N.A Plant DNA Kit , Promega , Cat#D3485-01.

Techniques: Recombinant, Magnetic Beads, Lysis, Labeling, Staining, Plasmid Preparation, DNA Library Preparation, Binding Assay, Methylated DNA Immunoprecipitation Sequencing, Software, Microscopy

Journal: ACS Omega

Article Title: Multiple Cross Displacement Amplification Combined with Real-Time Fluorescence Monitoring for Efficient, Specific, and Sensitive Neisseria meningitidis Detection

doi: 10.1021/acsomega.4c07249

Figure Lengend Snippet: Diagrammatic representation of the NM-RT-MCDA assay’s foundation. (A) The study’s primers’ locations and sequences that target the N. meningitis ctrA gene. Sense and complementary sequences are indicated by the right and left arrows, respectively. The primer positions are indicated by the yellow colored text. There are six amplification primers (C1, C2, D1, D2, R1 and R2), two cross primers (CP1 and CP2), and two displacement primers (F1 and F2). (B) MCDA reactions schematic diagram using the modified primers. A quencher (BHQ1, gray ball) was inserted in the middle of primer C1, which was extended with an endonuclease recognition site at the 5′ end and a fluorophore (FAM, yellow ball) at the 5′ end. Double-stranded target amplification containing the restriction endonuclease recognition site were produced following amplification. The fluorescent signal was produced upon recognition and cleavage by restriction endonuclease. (C) The NM-RT-MCDA detection system in its entire form. The entire procedure, which includes real-time fluorescence detection, MCDA reaction, endonuclease cleavage, and real-time DNA extraction.

Article Snippet: The DNA extraction kits were obtained from Beijing Transgen Biotech Co., Ltd. (Beijing, China).

Techniques: Amplification, Modification, Produced, Fluorescence, DNA Extraction

Journal: Computational and Mathematical Methods in Medicine

Article Title: An Investigation into the Correlation of Intestinal Flora with Obesity and Gestational Diabetes Mellitus

doi: 10.1155/2022/5677073

Figure Lengend Snippet: The details of main experimental reagents.

Article Snippet: DNA Extraction Kits , OMEGA-Soil DNA Kit , Omega Bio-Tek , USA.

Techniques: DNA Extraction, Gel Extraction, Sample Prep